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A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
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Immunologie et infection
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Journal JoVE Immunologie et infection
A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

10,184 Views

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08:43 min

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March 10, 2017

DOI:

10.3791/55199-v

DOI:
10.3791/55199-v

•

08:43 min
March 10, 2017

•

4 Views
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1Department of Microbiology, Infectiology and Immunology,Université de Montréal, 2Department of Pharmacology and Physiology,Université de Montréal, 3Molecular Biology Program,Université de Montréal

Summary

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We present in the current study a novel fluorescence-based assay using lymphocytes derived from a transgenic mouse. This assay is suitable for high-throughput screening (HTS) of small molecules endowed with the capacity of either inhibiting or promoting lymphocyte activation.

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Cite this Article

Fouda, A., Tahsini, M., Khodayarian, F., Al-nafisah, F., Rafei, M. A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules. J. Vis. Exp. (121), e55199, doi:10.3791/55199 (2017).

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