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A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
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A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
Capítulos
- 00:05Título
- 00:40Generation of Splenocyte Cell Suspension from Nur77GFP Mouse Spleens
- 02:07T Cell Isolation from the Splenocyte Cell Suspension
- 03:26T-cell Activation and the Induction of GFP Expression
- 04:42High Throughput Screening of Small Molecule
- 06:05Results: T and B Cell Receptors Activation Flowcytometry Results and a Representative T Cell HTS of Smal Molecules
- 07:56Conclusion
We present in the current study a novel fluorescence-based assay using lymphocytes derived from a transgenic mouse. This assay is suitable for high-throughput screening (HTS) of small molecules endowed with the capacity of either inhibiting or promoting lymphocyte activation.
