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实验室规模的生产和治疗性抗体的纯化
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Biyokimya
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JoVE Journal Biyokimya
Laboratory Scale Production and Purification of a Therapeutic Antibody
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实验室规模的生产和治疗性抗体的纯化

16,927 Views

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09:54 min

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January 24, 2017

DOI:

10.3791/55153-v

DOI:
10.3791/55153-v

•

09:54 min
January 24, 2017

•

13 Views
, , , ,
1Faculty of Pharmacy,University of Sydney

Özet

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这个协议描述在哺乳动物表达系统中生产的治疗性抗体的。所描述的方法包括制备载体DNA，稳定转染和人胚肾293细胞系的无血清适应的，设置大规模培养和纯化使用亲和层析组成。

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Cite this Article

Elgundi, Z., Sifniotis, V., Reslan, M., Cruz, E., Kayser, V. Laboratory Scale Production and Purification of a Therapeutic Antibody. J. Vis. Exp. (119), e55153, doi:10.3791/55153 (2017).

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