Dergi
/
Nörobilim
/
Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions
JoVE Journal
Nörobilim
Author Produced
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Nörobilim
Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions

Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions

DOI:
10.3791/55108-v

•

15:08 min

•

October 21, 2017

•

, , , ,
1Department of Neuroscience, School of Medicine,Universidad Central del Caribe, 2Department of Biochemistry, School of Medicine,Universidad Central del Caribe, 3Department of Pharmacology and Toxicology, Medical Sciences, Campus,University of Puerto Rico

Bölümler

  • 00:00Başlık
  • 01:01Isolation of Primary Astrocytes from Rat Cortices
  • 09:25Oxygen-Glucose Deprivation (OGD) of Primary Astrocytes
  • 13:40Representative Results
  • 14:08Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Ischemic stroke is a complex event in which the specific contribution of astrocytes to the affected brain region exposed to oxygen glucose deprivation (OGD) is difficult to study. This article introduces a methodology to obtain isolated astrocytes and study their reactivity and proliferation under OGD conditions.

Etiketler

AstrocyteAstrocyte ReactivityAstrocyte ProliferationIn VitroIschemic-like ConditionsOxygen Glucose Deprivation (OGD)GFAPPrimary Astrocytic Cell CulturePup Rat CorticesCerebral HemispheresCorticesMeningesDissection MicroscopeComplete DMEM Media

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5

Conditions Affecting Social Space in Drosophila melanogaster

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

A Protocol for Measuring Cue Reactivity in a Rat Model of Cocaine Use Disorder

Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation

A Novel In Vitro Live-imaging Assay of Astrocyte-mediated Phagocytosis Using pH Indicator-conjugated Synaptosomes

Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One’s Thumb

Levator Auris Longus Preparation for Examination of Mammalian Neuromuscular Transmission Under Voltage Clamp Conditions

Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase

Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis

Read Article