Dergi
/
Nörobilim
/
Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions
JoVE Journal
Nörobilim
Author Produced
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Nörobilim
Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions

Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions

9,645 Views

•

15:08 min

•

October 21, 2017

DOI:

10.3791/55108-v

DOI:
10.3791/55108-v

•

15:08 min
October 21, 2017

•

3 Views
, , , ,
1Department of Neuroscience, School of Medicine,Universidad Central del Caribe, 2Department of Biochemistry, School of Medicine,Universidad Central del Caribe, 3Department of Pharmacology and Toxicology, Medical Sciences, Campus,University of Puerto Rico

Özet

Automatically generated

Ischemic stroke is a complex event in which the specific contribution of astrocytes to the affected brain region exposed to oxygen glucose deprivation (OGD) is difficult to study. This article introduces a methodology to obtain isolated astrocytes and study their reactivity and proliferation under OGD conditions.

Explore More Videos

AstrocyteAstrocyte ReactivityAstrocyte ProliferationIn VitroIschemic-like ConditionsOxygen Glucose Deprivation (OGD)GFAPPrimary Astrocytic Cell CulturePup Rat CorticesCerebral HemispheresCorticesMeningesDissection MicroscopeComplete DMEM Media

Read Article

Cite this Article

Ferrer-Acosta, Y., Gonzalez-Vega, M. N., Rivera-Aponte, D. E., Martinez-Jimenez, S. M., Martins, A. H. Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions. J. Vis. Exp. (128), e55108, doi:10.3791/55108 (2017).

Download .ris file

Copy

Share Video

.

Copy