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Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions
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JoVE Journal Neurowissenschaften
Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions

Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions

9,645 Views

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15:08 min

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October 21, 2017

DOI:

10.3791/55108-v

DOI:
10.3791/55108-v

•

15:08 min
October 21, 2017

•

3 Views
, , , ,
1Department of Neuroscience, School of Medicine,Universidad Central del Caribe, 2Department of Biochemistry, School of Medicine,Universidad Central del Caribe, 3Department of Pharmacology and Toxicology, Medical Sciences, Campus,University of Puerto Rico

Summary

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Ischemic stroke is a complex event in which the specific contribution of astrocytes to the affected brain region exposed to oxygen glucose deprivation (OGD) is difficult to study. This article introduces a methodology to obtain isolated astrocytes and study their reactivity and proliferation under OGD conditions.

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Cite this Article

Ferrer-Acosta, Y., Gonzalez-Vega, M. N., Rivera-Aponte, D. E., Martinez-Jimenez, S. M., Martins, A. H. Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions. J. Vis. Exp. (128), e55108, doi:10.3791/55108 (2017).

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