Journal
/
Neurowissenschaften
/
Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions
JoVE Journal
Neurowissenschaften
Author Produced
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Neurowissenschaften
Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions

Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions

DOI:
10.3791/55108-v

•

15:08 min

•

October 21, 2017

•

, , , ,
1Department of Neuroscience, School of Medicine,Universidad Central del Caribe, 2Department of Biochemistry, School of Medicine,Universidad Central del Caribe, 3Department of Pharmacology and Toxicology, Medical Sciences, Campus,University of Puerto Rico

Kapitel

  • 00:00Titel
  • 01:01Isolation of Primary Astrocytes from Rat Cortices
  • 09:25Oxygen-Glucose Deprivation (OGD) of Primary Astrocytes
  • 13:40Representative Results
  • 14:08Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Ischemic stroke is a complex event in which the specific contribution of astrocytes to the affected brain region exposed to oxygen glucose deprivation (OGD) is difficult to study. This article introduces a methodology to obtain isolated astrocytes and study their reactivity and proliferation under OGD conditions.

Tags

AstrocyteAstrocyte ReactivityAstrocyte ProliferationIn VitroIschemic-like ConditionsOxygen Glucose Deprivation (OGD)GFAPPrimary Astrocytic Cell CulturePup Rat CorticesCerebral HemispheresCorticesMeningesDissection MicroscopeComplete DMEM Media

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5

Conditions Affecting Social Space in Drosophila melanogaster

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

A Protocol for Measuring Cue Reactivity in a Rat Model of Cocaine Use Disorder

Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation

A Novel In Vitro Live-imaging Assay of Astrocyte-mediated Phagocytosis Using pH Indicator-conjugated Synaptosomes

Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One’s Thumb

Levator Auris Longus Preparation for Examination of Mammalian Neuromuscular Transmission Under Voltage Clamp Conditions

Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase

Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis

Read Article