Dergi
/
İmmünoloji ve Enfeksiyon
/
Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells
JoVE Journal
İmmünoloji ve Enfeksiyon
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal İmmünoloji ve Enfeksiyon
Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells

Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells

8,635 Views

•

08:10 min

•

February 06, 2017

DOI:

10.3791/55038-v

DOI:
10.3791/55038-v

•

08:10 min
February 06, 2017

•

3 Views
, , ,
1Department of Microbiology and Immunology,Miller School of Medicine, University of Miami, 2Division of Infectious Diseases,Miller School of Medicine, University of Miami

Özet

Automatically generated

We describe here an isolation method to obtain human endocervical intraepithelial lymphocytes for the analysis of intraepithelial gamma delta T cells. This protocol can be extended for the purification of endocervical gamma delta T cells by magnetic beads or by cell sorting.

Explore More Videos

EndocervicalGamma Delta T CellsFlow CytometryIsolationCharacterizationIntraepithelialImmune EventsCytobrushIMDM MediumCell StrainerFlow Cytometry AnalysisAntibodiesViability DyeCompensation ControlsMagnetic Bead IsolationTCR Gamma Delta

Read Article

Cite this Article

Strbo, N., Romero, L., Alcaide, M., Fischl, M. Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells. J. Vis. Exp. (120), e55038, doi:10.3791/55038 (2017).

Download .ris file

Copy

Share Video

.

Copy