Dergi
/
Biyokimya
/
وسيلة فعالة لعزل الحمض النووي الريبي العالية النقاء من بذور للاستخدام في التحليل الكمي Transcriptome على
JoVE Journal
Biyokimya
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Biyokimya
An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
Please note that all translations are automatically generated. Click here for the English version.

وسيلة فعالة لعزل الحمض النووي الريبي العالية النقاء من بذور للاستخدام في التحليل الكمي Transcriptome على

9,447 Views

•

06:31 min

•

January 11, 2017

DOI:

10.3791/55008-v

DOI:
10.3791/55008-v

•

06:31 min
January 11, 2017

•

3 Views
, ,
1Laboratory of Biological Diversity, Department of Evolutionary Biology and Biodiversity,National Institute for Basic Biology, 2Department of Basic Biology,SOKENDAI (The Graduate University for Advanced Studies), 3Department of Cell Biology,National Institute for Basic Biology

Özet

Automatically generated

We have succeeded in establishing a method for RNA isolation from plant seeds containing large amounts of oils, proteins, and polyphenols, which have inhibitory effects on high-purity RNA isolation. Our method is suitable for monitoring the expression of genes with low level transcripts in seeds.

Explore More Videos

RNA ExtractionSeedQuantitative Transcriptome AnalysisArabidopsis ThalianaPVPCell Lysis BufferCentrifugationHomogenizationSpin Column-based MethodHigh-purity RNACereal BiologyGene ProfilesTranscripts

Read Article

Cite this Article

Kanai, M., Mano, S., Nishimura, M. An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis. J. Vis. Exp. (119), e55008, doi:10.3791/55008 (2017).

Download .ris file

Copy

Share Video

.

Copy