Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
19,884 Views
•
09:06 min
•
December 23, 2016
DOI:
Özet
Automatically generated
Here, we present a protocol to site-specifically introduce chemical probes into an antibody fragment by genetically incorporating an azide-containing amino acid, and subsequently coupling the azide with a chemical probe by strain-promoted azide-alkyne cycloaddition (SPAAC).
Read Article
Cite this Article
Kim, S., Ko, W., Park, H., Lee, H. S. Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition. J. Vis. Exp. (118), e54922, doi:10.3791/54922 (2016).
Copy