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Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
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Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
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December 23, 2016
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Here, we present a protocol to site-specifically introduce chemical probes into an antibody fragment by genetically incorporating an azide-containing amino acid, and subsequently coupling the azide with a chemical probe by strain-promoted azide-alkyne cycloaddition (SPAAC).
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Kim, S., Ko, W., Park, H., Lee, H. S. Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition. J. Vis. Exp. (118), e54922, doi:10.3791/54922 (2016).
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