Dergi
/
Gelişim Biyolojisi
/
Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays
JoVE Journal
Gelişim Biyolojisi
This content is Free Access.
JoVE Journal Gelişim Biyolojisi
Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

26,174 Views

•

09:20 min

•

January 08, 2017

DOI:

10.3791/54900-v

DOI:
10.3791/54900-v

•

09:20 min
January 08, 2017

•

24 Views
, , , , , , , ,
1Department of Cognitive Neurosciences,Radboudumc, 2Donders Institute for Brain, Cognition and Behaviour,Radboud University, 3Department of Human Genetics,Radboudumc, 4Department of Molecular Developmental Biology,Radboud University

Özet

Automatically generated

We modify and implement a previously published protocol describing the rapid, reproducible, and efficient differentiation of human induced Pluripotent Stem Cells (hiPSCs) into excitatory cortical neurons12. Specifically, our modification allows for control of neuronal cell density and use on micro-electrode arrays to measure electrophysiological properties at the network level.

Explore More Videos

Neuronal DifferentiationInduced Pluripotent Stem CellsMicro-electrode ArraysNeurological DisordersNeurobiologyRapid DifferentiationDMEM/F12CDSE8 MediumDoxycyclineROCK InhibitorHiPSCsCell SuspensionLamininMEAsCell Plating

Read Article

Cite this Article

Frega, M., van Gestel, S. H. C., Linda, K., van der Raadt, J., Keller, J., Van Rhijn, J., Schubert, D., Albers, C. A., Nadif Kasri, N. Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays. J. Vis. Exp. (119), e54900, doi:10.3791/54900 (2017).

Download .ris file

Copy

Share Video

.

Copy