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Biologia do Desenvolvimento
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Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

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09:20 min

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January 08, 2017

DOI:

10.3791/54900-v

DOI:
10.3791/54900-v

•

09:20 min
January 08, 2017

•

24 Views
, , , , , , , ,
1Department of Cognitive Neurosciences,Radboudumc, 2Donders Institute for Brain, Cognition and Behaviour,Radboud University, 3Department of Human Genetics,Radboudumc, 4Department of Molecular Developmental Biology,Radboud University

Summary

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We modify and implement a previously published protocol describing the rapid, reproducible, and efficient differentiation of human induced Pluripotent Stem Cells (hiPSCs) into excitatory cortical neurons12. Specifically, our modification allows for control of neuronal cell density and use on micro-electrode arrays to measure electrophysiological properties at the network level.

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Frega, M., van Gestel, S. H. C., Linda, K., van der Raadt, J., Keller, J., Van Rhijn, J., Schubert, D., Albers, C. A., Nadif Kasri, N. Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays. J. Vis. Exp. (119), e54900, doi:10.3791/54900 (2017).

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