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Biologia do Desenvolvimento
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Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

DOI:
10.3791/54900-v

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09:20 min

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January 08, 2017

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, , , , , , , ,
1Department of Cognitive Neurosciences,Radboudumc, 2Donders Institute for Brain, Cognition and Behaviour,Radboud University, 3Department of Human Genetics,Radboudumc, 4Department of Molecular Developmental Biology,Radboud University

Capítulos

  • 00:05Título
  • 00:41Plating hiPSCs
  • 03:42Adding Rat Astrocytes
  • 05:48Characterization of Neuronal Network Activity Using MEAs
  • 06:31Results: Characterization of hiPSC-derived Neurons
  • 08:21Conclusion

Summary

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We modify and implement a previously published protocol describing the rapid, reproducible, and efficient differentiation of human induced Pluripotent Stem Cells (hiPSCs) into excitatory cortical neurons12. Specifically, our modification allows for control of neuronal cell density and use on micro-electrode arrays to measure electrophysiological properties at the network level.

Tags

Keywords: Neuronal DifferentiationInduced Pluripotent Stem CellsMicro-electrode ArraysNeurological DisordersNeurobiologyRapid DifferentiationDMEM/F12CDSE8 MediumDoxycyclineROCK InhibitorHiPSCsCell SuspensionLamininMEAsCell Plating

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