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Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
JoVE Journal
Genetik
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JoVE Journal Genetik
Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

10,764 Views

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10:23 min

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January 19, 2017

DOI:

10.3791/54858-v

DOI:
10.3791/54858-v

•

10:23 min
January 19, 2017

•

4 Views
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1Unit for Lymphopoieseis, Immunology Department, INSERM U1223, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur,Pasteur Enstitüsü, 2Center for Translational Science,Institut Pasteur, INSERM UMS20

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This protocol describes how to assess the expression of a large array of genes at the clonal level. Single-cell RT-qPCR produces highly reliable results with a strong sensitivity for hundreds of samples and genes.

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Single-cell Gene ExpressionMultiplex RT-qPCRHeterogeneityRare Lymphoid PopulationsPre-amplificationAssay MixFACSInnate Lymphoid CellsNo-input ControlCDNA DilutionReverse TranscriptionPre-amplification

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Perchet, T., Chea, S., Hasan, M., Cumano, A., Golub, R. Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations. J. Vis. Exp. (119), e54858, doi:10.3791/54858 (2017).

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