Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Thibaut Perchet; Sylvestre Chea; Milena Hasan; Ana Cumano; Rachel Golub

doi:10.3791/54858

Journal

/

Génétique

/

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Journal JoVE
Génétique

Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.

Journal JoVE Génétique

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

10,764 Views

•

10:23 min

•

January 19, 2017

DOI:

10.3791/54858-v

DOI:

10.3791/54858-v

•

10:23 min

January 19, 2017

•

4 Views

Thibaut Perchet¹, Sylvestre Chea¹, Milena Hasan², Ana Cumano¹, Rachel Golub¹

¹Unit for Lymphopoieseis, Immunology Department, INSERM U1223, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur,Institut Pasteur, ²Center for Translational Science,Institut Pasteur, INSERM UMS20

Summary

This protocol describes how to assess the expression of a large array of genes at the clonal level. Single-cell RT-qPCR produces highly reliable results with a strong sensitivity for hundreds of samples and genes.

Explore More Videos

Single-cell Gene Expression Multiplex RT-qPCR Heterogeneity Rare Lymphoid Populations Pre-amplification Assay Mix FACS Innate Lymphoid Cells No-input Control CDNA Dilution Reverse Transcription Pre-amplification

Read Article

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Summary

Automatically generated

Explore More Videos