Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Measurement of Differentially Methylated INS DNA Species in Human Serum Samples as a Biomarker of Islet β Cell Death
6,324 Views
•
10:34 min
•
December 21, 2016
DOI:
1Department of Pediatrics, IU Center for Diabetes and Metabolic Disease,Indiana University School of Medicine, 2Department of Pediartics, Omaha Children’s Hospital and Medical Center,University of Nebraska Medical Center, 3Departments of Biochemistry and Molecular Biology, Medicine, and Cellular and Integrative Physiology, IU Center for Diabetes and Metabolic Disease,Indiana University School of Medicine, 4Indiana Biosciences Research Institute
Özet
Automatically generated
Islet β cell death precedes development of type 1 diabetes, and detecting this process may allow for early therapeutic intervention. Here, we provide a detailed description of how to measure differentially methylated INS DNA species in human serum as a biomarker of β cell death.
Read Article
Cite this Article
Tersey, S. A., Nelson, J. B., Fisher, M. M., Mirmira, R. G. Measurement of Differentially Methylated INS DNA Species in Human Serum Samples as a Biomarker of Islet β Cell Death. J. Vis. Exp. (118), e54838, doi:10.3791/54838 (2016).
Copy