Measurement of Differentially Methylated <em>INS</em> DNA Species in Human Serum Samples as a Biomarker of Islet &#946; Cell Death

Sarah A Tersey; Jennifer B Nelson; Marisa M Fisher; Raghavendra G Mirmira

doi:10.3791/54838

ジャーナル

/

遺伝学

/

Measurement of Differentially Methylated INS DNA Species in Human Serum Samples as a Biomarker of Islet β Cell Death

JoVE Journal
遺伝学

このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。

JoVE Journal 遺伝学

Measurement of Differentially Methylated INS DNA Species in Human Serum Samples as a Biomarker of Islet β Cell Death

Measurement of Differentially Methylated INS DNA Species in Human Serum Samples as a Biomarker of Islet β Cell Death

DOI:

10.3791/54838-v

•

10:34 min

•

December 21, 2016

•

Sarah A Tersey, Jennifer B Nelson, Marisa M Fisher, Raghavendra G Mirmira^3,4

¹Department of Pediatrics, IU Center for Diabetes and Metabolic Disease,Indiana University School of Medicine, ²Department of Pediartics, Omaha Children’s Hospital and Medical Center,University of Nebraska Medical Center, ³Departments of Biochemistry and Molecular Biology, Medicine, and Cellular and Integrative Physiology, IU Center for Diabetes and Metabolic Disease,Indiana University School of Medicine, ⁴Indiana Biosciences Research Institute

章

00:05標題
00:42Serum DNA Extraction
03:01Bisulfite Conversion
04:59Multiplex Digital PCR
08:00Results: Differentially Methylated INS DNA Measurements
09:25Conclusion

概要

自動翻訳

Islet β cell death precedes development of type 1 diabetes, and detecting this process may allow for early therapeutic intervention. Here, we provide a detailed description of how to measure differentially methylated INS DNA species in human serum as a biomarker of β cell death.

Article

Embed

プレイリスト追加

Usage Statistics

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Generation of Fluorescent Protein Fusions in Candida Species

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples