Dergi
/
Genetik
/
Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts
JoVE Journal
Genetik
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Genetik
Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts

Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts

12,498 Views

•

11:11 min

•

December 17, 2016

DOI:

10.3791/54787-v

DOI:
10.3791/54787-v

•

11:11 min
December 17, 2016

•

3 Views
, , , , , ,
1Department of Cardiology,Boston Children’s Hospital, 2Pediatri Anabilim Dalı,Harvard Tıp Fakültesi, 3Genetik Bölümü,Harvard Tıp Fakültesi, 4Howard Hughes Medical Institute

Özet

Automatically generated

In this manuscript, a method to prepare recombinant adeno-associated virus 9 (rAAV9) vectors to manipulate gene expression in the mouse heart is described.

Explore More Videos

RAAV9Gene ExpressionMouse HeartIn VivoGene FunctionCardiologyMolecular GeneticsGene ManipulationHEK293 CellsTransfectionPEICell CultureCell HarvestingCell LysisIodixanol GradientVirus Purification

Read Article

Cite this Article

Ding, J., Lin, Z., Jiang, J., Seidman, C. E., Seidman, J. G., Pu, W. T., Wang, D. Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts. J. Vis. Exp. (118), e54787, doi:10.3791/54787 (2016).

Download .ris file

Copy

Share Video

.

Copy