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Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts
JoVE Journal
Genetik
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JoVE Journal Genetik
Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts

Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts

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11:11 min

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December 17, 2016

DOI:

10.3791/54787-v

DOI:
10.3791/54787-v

•

11:11 min
December 17, 2016

•

3 Views
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1Department of Cardiology,Boston Children’s Hospital, 2Pediatri Anabilim Dalı,Harvard Tıp Fakültesi, 3Genetik Bölümü,Harvard Tıp Fakültesi, 4Howard Hughes Medical Institute

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In this manuscript, a method to prepare recombinant adeno-associated virus 9 (rAAV9) vectors to manipulate gene expression in the mouse heart is described.

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Cite this Article

Ding, J., Lin, Z., Jiang, J., Seidman, C. E., Seidman, J. G., Pu, W. T., Wang, D. Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts. J. Vis. Exp. (118), e54787, doi:10.3791/54787 (2016).

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