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Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts
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JoVE Journal 遺伝学
Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts

Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts

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11:11 min

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December 17, 2016

DOI:

10.3791/54787-v

DOI:
10.3791/54787-v

•

11:11 min
December 17, 2016

•

3 Views
, , , , , ,
1Department of Cardiology,Boston Children’s Hospital, 2小児科,Harvard Medical School, 3遺伝学科,Harvard Medical School, 4Howard Hughes Medical Institute

概要

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In this manuscript, a method to prepare recombinant adeno-associated virus 9 (rAAV9) vectors to manipulate gene expression in the mouse heart is described.

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Cite this Article

Ding, J., Lin, Z., Jiang, J., Seidman, C. E., Seidman, J. G., Pu, W. T., Wang, D. Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts. J. Vis. Exp. (118), e54787, doi:10.3791/54787 (2016).

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