Dergi
/
Nörobilim
/
The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions
JoVE Journal
Nörobilim
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Nörobilim
The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

DOI:
10.3791/54757-v

•

11:08 min

•

November 14, 2016

•

, , ,
1Department of Cell Morphology and Molecular Neurobiology, Faculty of Biology and Biotechnology,Ruhr-University

Bölümler

  • 00:05Başlık
  • 02:01Preparation and Cultivation of Primary Cortical Astrocytes
  • 05:19Preparing Astrocytes for the Indirect Coculture
  • 06:48Preparation of Primary Hippocampal Neurons
  • 09:03Results: The Indirect, Astrocyte-neuron Coculture System Allows for Separate Analysis of Astrocytes, Neurons and Secreted Molecular Mediators
  • 10:01Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This protocol describes the indirect neuron-astrocyte coculture for compartmentalized analysis of neuron-glia interactions.

Etiketler

Indirect Neuron-astrocyte Coculture AssayNeuron-glia InteractionsSynapse FormationAstrocyte SecretomePrimary AstrocytesPrimary Embryonic NeuronsPermeable Membrane

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Brain Imaging Investigation of the Neural Correlates of Observing Virtual Social Interactions

Brain Imaging Investigation of the Memory-Enhancing Effect of Emotion

Brain Imaging Investigation of the Impairing Effect of Emotion on Cognition

An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices

An In Vitro Model for the Study of Cellular Pathophysiology in Globoid Cell Leukodystrophy

In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening

Read Article