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The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions
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JoVE Journal 神経科学
The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

DOI:
10.3791/54757-v

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11:08 min

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November 14, 2016

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, , ,
1Department of Cell Morphology and Molecular Neurobiology, Faculty of Biology and Biotechnology,Ruhr-University

章

  • 00:05標題
  • 02:01Preparation and Cultivation of Primary Cortical Astrocytes
  • 05:19Preparing Astrocytes for the Indirect Coculture
  • 06:48Preparation of Primary Hippocampal Neurons
  • 09:03Results: The Indirect, Astrocyte-neuron Coculture System Allows for Separate Analysis of Astrocytes, Neurons and Secreted Molecular Mediators
  • 10:01Conclusion

概要

自動翻訳

自動翻訳

This protocol describes the indirect neuron-astrocyte coculture for compartmentalized analysis of neuron-glia interactions.

タグ

Indirect Neuron-astrocyte Coculture AssayNeuron-glia InteractionsSynapse FormationAstrocyte SecretomePrimary AstrocytesPrimary Embryonic NeuronsPermeable Membrane

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