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Automated Robotic Liquid Handling Assembly of Modular DNA Devices
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Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

12,013 Views

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11:22 min

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December 01, 2017

DOI:

10.3791/54703-v

DOI:
10.3791/54703-v

•

11:22 min
December 01, 2017

•

5 Views
, , , ,
1Graduate Program in Molecular Biology, Cell Biology, and Biochemistry,Boston University, 2Biological Design Center,Boston University, 3Lattice Automation, 4Department of Electrical and Computer Engineering, Biological Design Center,Boston University

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Here, an automated workflow to perform modular DNA "device" assembly using a modular cloning DNA assembly method on liquid-handling robots is presented. The protocol uses an intuitive software tool for generating liquid handler picklists for combinatorial DNA device library generation, which we demonstrate using two liquid handling platforms.

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Ortiz, L., Pavan, M., McCarthy, L., Timmons, J., Densmore, D. M. Automated Robotic Liquid Handling Assembly of Modular DNA Devices. J. Vis. Exp. (130), e54703, doi:10.3791/54703 (2017).

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