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Automated Robotic Liquid Handling Assembly of Modular DNA Devices
JoVE Revista
Bioingeniería
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JoVE Revista Bioingeniería
Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

12,032 Views

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11:22 min

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December 01, 2017

DOI:

10.3791/54703-v

DOI:
10.3791/54703-v

•

11:22 min
December 01, 2017

•

5 Views
, , , ,
1Graduate Program in Molecular Biology, Cell Biology, and Biochemistry,Boston University, 2Biological Design Center,Boston University, 3Lattice Automation, 4Department of Electrical and Computer Engineering, Biological Design Center,Boston University

Summary

Automatically generated

Here, an automated workflow to perform modular DNA "device" assembly using a modular cloning DNA assembly method on liquid-handling robots is presented. The protocol uses an intuitive software tool for generating liquid handler picklists for combinatorial DNA device library generation, which we demonstrate using two liquid handling platforms.

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Cite this Article

Ortiz, L., Pavan, M., McCarthy, L., Timmons, J., Densmore, D. M. Automated Robotic Liquid Handling Assembly of Modular DNA Devices. J. Vis. Exp. (130), e54703, doi:10.3791/54703 (2017).

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