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Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues

DOI:
10.3791/54485-v

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09:27 min

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February 17, 2017

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, , , , , ,
1Department of Chemistry and Biochemistry,Central Michigan University, 2Kimya Bölümü,University of Southern Maine

Bölümler

  • 00:05Başlık
  • 01:14Expression and Purification of Trehalose Synthase Thermoproteus tenax (TreT)
  • 04:04Synthesis and Purification of a Trehalose Analogue — Exemplified with the Synthesis of 6-Azido-trehalose (6-TreAz)
  • 06:59Results: One-step Synthesis of Trehalose Analogues Using TreT
  • 08:14Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Trehalose analogues are emerging as important molecules for bio(techno)logical and biomedical applications. We describe an optimized protocol for enzymatically synthesizing and purifying trehalose analogues that is simple, efficient, fast, and environmentally friendly. Its application to the rapid production and administration of a probe for the detection of mycobacteria is demonstrated.

Etiketler

Trehalose AnaloguesEnzymatic SynthesisAll-aqueous PurificationTreT ExpressionE. ColiTuberculosis DetectionBiopreservationNoncaloric Sweeteners

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