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Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
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生化学
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JoVE Journal 生化学
Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues

10,190 Views

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09:27 min

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February 17, 2017

DOI:

10.3791/54485-v

DOI:
10.3791/54485-v

•

09:27 min
February 17, 2017

•

3 Views
, , , , , ,
1Department of Chemistry and Biochemistry,Central Michigan University, 2化学科,University of Southern Maine

概要

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Trehalose analogues are emerging as important molecules for bio(techno)logical and biomedical applications. We describe an optimized protocol for enzymatically synthesizing and purifying trehalose analogues that is simple, efficient, fast, and environmentally friendly. Its application to the rapid production and administration of a probe for the detection of mycobacteria is demonstrated.

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Cite this Article

Meints, L. M., Poston, A. W., Piligian, B. F., Olson, C. D., Badger, K. S., Woodruff, P. J., Swarts, B. M. Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues. J. Vis. Exp. (120), e54485, doi:10.3791/54485 (2017).

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