/
/
Patrones sustractiva rápida de las capas de células vivas con una sonda de microfluidos
This content is Free Access.
Patrones sustractiva rápida de las capas de células vivas con una sonda de microfluidos
Bölümler
- 00:05Başlık
- 01:16Microfluidic Probe (MFP) Head and Platform Cleaning and Preparation
- 06:13Creating the Hierarchical Hydrodynamic Flow Confinement (hHFC) and Patterning Cell Monolayers Using hHFC
- 08:10Downstream Processing for Sampling and DNA Amplification
- 09:46Results: Rapid Subtractive Patterning of Cell Monolayers Using a Hierachical HFC
- 10:54Conclusion
We present a protocol to perform subtractive patterning of live cell monolayers on a surface. This is achieved by local and selective lysis of adherent cells using a microfluidic probe (MFP). The cell lysate retrieved from local regions can be used for downstream analysis, enabling molecular profiling studies.
