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Rapid Subtractive Patterning of Live Cell Layers with a Microfluidic Probe
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September 15, 2016
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We present a protocol to perform subtractive patterning of live cell monolayers on a surface. This is achieved by local and selective lysis of adherent cells using a microfluidic probe (MFP). The cell lysate retrieved from local regions can be used for downstream analysis, enabling molecular profiling studies.
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Kashyap, A., Cors, J. F., Lovchik, R. D., Kaigala, G. V. Rapid Subtractive Patterning of Live Cell Layers with a Microfluidic Probe. J. Vis. Exp. (115), e54447, doi:10.3791/54447 (2016).
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