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Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells
JoVE Journal
İmmünoloji ve Enfeksiyon
This content is Free Access.
JoVE Journal İmmünoloji ve Enfeksiyon
Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells

Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells

DOI:

09:25 min

August 19, 2016

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Bölümler

  • 00:05Başlık
  • 00:58Bacterial Culture
  • 01:48Host Cell Infection with Permeable Membrane Insert System
  • 03:52Sample Collection
  • 06:13Results: Effect of Secreted Group A Streptococcus Toxins on Host Cells Using a Permeable Membrane Insert-based Infection System
  • 07:56Conclusion

Özet

Otomatik Çeviri

Here, a method using a permeable membrane insert-based infection system to study the effects of Streptolysin S, a secreted toxin produced by Group A Streptococcus, on keratinocytes is described. This system can be readily applied to the study of other secreted bacterial proteins on various host cell types during infection.

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