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Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells
Chapters
- 00:05Title
- 00:58Bacterial Culture
- 01:48Host Cell Infection with Permeable Membrane Insert System
- 03:52Sample Collection
- 06:13Results: Effect of Secreted Group A Streptococcus Toxins on Host Cells Using a Permeable Membrane Insert-based Infection System
- 07:56Conclusion
Here, a method using a permeable membrane insert-based infection system to study the effects of Streptolysin S, a secreted toxin produced by Group A Streptococcus, on keratinocytes is described. This system can be readily applied to the study of other secreted bacterial proteins on various host cell types during infection.
