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Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
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JoVE Journal Biyokimya
Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

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10:53 min

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January 16, 2017

DOI:

10.3791/54391-v

DOI:
10.3791/54391-v

•

10:53 min
January 16, 2017

•

3 Views
, , , ,
1Department of Veterinary & Biomedical Sciences,University of Minnesota, 2Department of Veterinary Biosciences,Ohio State University, 3School of Biotechnology,Gautam Buddha University

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This manuscript describes an approach to isolate select cognate RNPs formed in eukaryotic cells via a specific oligonucleotide-directed enrichment. We demonstrate the applicability of this approach by isolating a cognate RNP bound to the retroviral 5' untranslated region that is composed of DHX9/RNA helicase A.

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Singh, G., Fritz, S. M., Ranji, A., Singh, D., Boris-Lawrie, K. Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution. J. Vis. Exp. (119), e54391, doi:10.3791/54391 (2017).

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