Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
8,809 Views
•
10:53 min
•
January 16, 2017
DOI:
Summary
Automatically generated
This manuscript describes an approach to isolate select cognate RNPs formed in eukaryotic cells via a specific oligonucleotide-directed enrichment. We demonstrate the applicability of this approach by isolating a cognate RNP bound to the retroviral 5' untranslated region that is composed of DHX9/RNA helicase A.
Read Article
Cite this Article
Singh, G., Fritz, S. M., Ranji, A., Singh, D., Boris-Lawrie, K. Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution. J. Vis. Exp. (119), e54391, doi:10.3791/54391 (2017).
Copy