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Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo
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Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

18,259 Views

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11:42 min

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June 19, 2016

DOI:

10.3791/54142-v

DOI:
10.3791/54142-v

•

11:42 min
June 19, 2016

•

9 Views
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1National Key Laboratory of Cognitive Neuroscience and Learning,Beijing Normal University, 2Department of Biomedical Science,The University of Sheffield

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Sharp microelectrodes enable accurate electrophysiological characterization of photoreceptor and visual interneuron output in living Drosophila. Here we show how to use this method to record high-quality voltage responses of individual cells to controlled light stimulation. This method is ideal for studying neural information processing in insect compound eyes.

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Juusola, M., Dau, A., Zheng, L., Rien, D. Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo. J. Vis. Exp. (112), e54142, doi:10.3791/54142 (2016).

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