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Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes
JoVE Journal
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Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes

Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes

DOI:
10.3791/54114-v

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08:14 min

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May 08, 2016

•

,
1Division of Infectious Diseases, Department of Medicine,Boston Children’s Hospital

'Bölümler'

  • 00:05'Başlık'
  • 00:39Preparation of a Single Cell Suspension from the Small Intestinal Intraepithelial Layer and Lamina Propria
  • 05:22Preparation of a Single Cell Suspension from Peyer’s Patches
  • 06:20Results: Intestinal Lymphocytes Have FSC and SSC Characteristics Similar to Splenocytes
  • 07:10Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

There is growing interest in the quantitative characterization of intestinal lymphocytes owing to increasing recognition that these cells play a critical role in a variety of intestinal and systemic diseases. In this protocol, we describe how to isolate single cell populations from different small-intestinal compartments for subsequent flow cytometric characterization.

'Etiketler'

MurineSmall IntestinalLymphocytesFlow CytometricIsolationCharacterizationMucosal ImmunologyMicrobiotaIntestinal Immune SystemLaparotomyPeritoneal CavitySmall IntestinePeyer’s PatchesLymphocytesIntestinal ContentsFat Removal

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