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Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes

Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes

26,709 Views

08:14 min

May 08, 2016

DOI:

08:14 min
May 08, 2016

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Summary

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There is growing interest in the quantitative characterization of intestinal lymphocytes owing to increasing recognition that these cells play a critical role in a variety of intestinal and systemic diseases. In this protocol, we describe how to isolate single cell populations from different small-intestinal compartments for subsequent flow cytometric characterization.

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