Dergi
/
Biyomühendislik
/
Руководство по Structured Illumination TIRF микроскопией на высокой скорости с несколькими цветами
JoVE Journal
Biyomühendislik
This content is Free Access.
JoVE Journal Biyomühendislik
A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
Please note that all translations are automatically generated. Click here for the English version.

Руководство по Structured Illumination TIRF микроскопией на высокой скорости с несколькими цветами

24,510 Views

•

11:15 min

•

May 30, 2016

DOI:

10.3791/53988-v

DOI:
10.3791/53988-v

•

11:15 min
May 30, 2016

•

20 Views
, ,
1Department of Chemical Engineering and Biotechnology,University of Cambridge

Özet

Automatically generated

This article provides an in depth guide for the assembly and operation of a structured illumination microscope operating with total internal reflection fluorescence illumination (TIRF-SIM) to image dynamic biological processes with optical super-resolution in multiple colors.

Explore More Videos

Structured Illumination MicroscopyTIRFHigh-speed ImagingMulti-colorCell BiologySuper-resolutionOptical AlignmentDichroic MirrorsSLMLaserObjective LensTube Lens

Read Article

Cite this Article

Young, L. J., Ströhl, F., Kaminski, C. F. A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors. J. Vis. Exp. (111), e53988, doi:10.3791/53988 (2016).

Download .ris file

Copy

Share Video

.

Copy