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A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
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A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
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May 30, 2016
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This article provides an in depth guide for the assembly and operation of a structured illumination microscope operating with total internal reflection fluorescence illumination (TIRF-SIM) to image dynamic biological processes with optical super-resolution in multiple colors.
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Young, L. J., Ströhl, F., Kaminski, C. F. A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors. J. Vis. Exp. (111), e53988, doi:10.3791/53988 (2016).
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