Journal
/
Biotechnik
/
A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
JoVE Journal
Biotechnik
This content is Free Access.
JoVE Journal Biotechnik
A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

24,489 Views

•

11:15 min

•

May 30, 2016

DOI:

10.3791/53988-v

DOI:
10.3791/53988-v

•

11:15 min
May 30, 2016

•

20 Views
, ,
1Department of Chemical Engineering and Biotechnology,University of Cambridge

Summary

Automatically generated

This article provides an in depth guide for the assembly and operation of a structured illumination microscope operating with total internal reflection fluorescence illumination (TIRF-SIM) to image dynamic biological processes with optical super-resolution in multiple colors.

Explore More Videos

Structured Illumination MicroscopyTIRFHigh-speed ImagingMulti-colorCell BiologySuper-resolutionOptical AlignmentDichroic MirrorsSLMLaserObjective LensTube Lens

Read Article

Cite this Article

Young, L. J., Ströhl, F., Kaminski, C. F. A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors. J. Vis. Exp. (111), e53988, doi:10.3791/53988 (2016).

Download .ris file

Copy

Share Video

.

Copy