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A Guide to Structured Illumination TIRF Microscopie op hoge snelheid met meerdere kleuren
JoVE Journal
Biyomühendislik
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JoVE Journal Biyomühendislik
A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
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A Guide to Structured Illumination TIRF Microscopie op hoge snelheid met meerdere kleuren

24,510 Views

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11:15 min

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May 30, 2016

DOI:

10.3791/53988-v

DOI:
10.3791/53988-v

•

11:15 min
May 30, 2016

•

20 Views
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1Department of Chemical Engineering and Biotechnology,University of Cambridge

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This article provides an in depth guide for the assembly and operation of a structured illumination microscope operating with total internal reflection fluorescence illumination (TIRF-SIM) to image dynamic biological processes with optical super-resolution in multiple colors.

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Young, L. J., Ströhl, F., Kaminski, C. F. A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors. J. Vis. Exp. (111), e53988, doi:10.3791/53988 (2016).

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