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Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

20,353 Views

06:51 min

April 29, 2016

DOI:

06:51 min
April 29, 2016

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Özet

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An ideal model for studying adult stem cell biology is the mouse hair follicle. Here we present a protocol for isolating different populations of hair follicles stem cells and epidermal keratinocytes, employing enzymatic digestion of mouse dorsal skin followed by FACS analysis.

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