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Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin
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JoVE Journal Gelişim Biyolojisi
Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

20,299 Views

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06:51 min

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April 29, 2016

DOI:

10.3791/53931-v

DOI:
10.3791/53931-v

•

06:51 min
April 29, 2016

•

49 Views
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1Department of Biology and Lokey Center, Laboratory of Stem Cell Biology and Regenerative Medicine,Technion Israel Institute of Technology, 2Strang Laboratory of Apoptosis and Cancer Biology,Howard Hughes Medical Institute, The Rockefeller University

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An ideal model for studying adult stem cell biology is the mouse hair follicle. Here we present a protocol for isolating different populations of hair follicles stem cells and epidermal keratinocytes, employing enzymatic digestion of mouse dorsal skin followed by FACS analysis.

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Soteriou, D., Kostic, L., Sedov, E., Yosefzon, Y., Steller, H., Fuchs, Y. Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin. J. Vis. Exp. (110), e53931, doi:10.3791/53931 (2016).

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