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Developmental Biology

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JoVE Journal Developmental Biology
Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin
 

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

Article DOI: 10.3791/53931-v • 06:51 min • April 29th, 2016
April 29th, 2016 •
1, 1, 1, 1, 2, 1
1Department of Biology and Lokey Center, Laboratory of Stem Cell Biology and Regenerative Medicine, Technion Israel Institute of Technology, 2Strang Laboratory of Apoptosis and Cancer Biology, Howard Hughes Medical Institute, The Rockefeller University

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An ideal model for studying adult stem cell biology is the mouse hair follicle. Here we present a protocol for isolating different populations of hair follicles stem cells and epidermal keratinocytes, employing enzymatic digestion of mouse dorsal skin followed by FACS analysis.

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Isolating Hair Follicle Stem Cells Epidermal Keratinocytes Dorsal Mouse Skin Cell Isolation Protocol Stem Cell Isolation Keratinocyte Isolation Heterogeneous Skin Cell Populations Fast And Reliable Technique Specific Cell Types Telogen Phase Of Hair Follicle Cycle Electric Clippers Disinfecting Skin Dissecting Pad Forceps Scissors Tissue Harvesting Dermis Clearance
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