Journal
/
Biología del desarrollo
/
Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin
JoVE Revista
Biología del desarrollo
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología del desarrollo
Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

20,338 Views

•

06:51 min

•

April 29, 2016

DOI:

10.3791/53931-v

DOI:
10.3791/53931-v

•

06:51 min
April 29, 2016

•

49 Views
, , , , ,
1Department of Biology and Lokey Center, Laboratory of Stem Cell Biology and Regenerative Medicine,Technion Israel Institute of Technology, 2Strang Laboratory of Apoptosis and Cancer Biology,Howard Hughes Medical Institute, The Rockefeller University

Summary

Automatically generated

An ideal model for studying adult stem cell biology is the mouse hair follicle. Here we present a protocol for isolating different populations of hair follicles stem cells and epidermal keratinocytes, employing enzymatic digestion of mouse dorsal skin followed by FACS analysis.

Explore More Videos

Hair Follicle Stem CellsEpidermal KeratinocytesDorsal Mouse SkinCell IsolationCell PopulationHair Follicle CycleSkin DissectionTrypsin DigestionHair Follicle Removal

Read Article

Cite this Article

Soteriou, D., Kostic, L., Sedov, E., Yosefzon, Y., Steller, H., Fuchs, Y. Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin. J. Vis. Exp. (110), e53931, doi:10.3791/53931 (2016).

Download .ris file

Copy

Share Video

.

Copy