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Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
JoVE Journal
Kimya
'Bu içeriği görüntülemek için JoVE aboneliği gereklidir.'  'Oturum açın veya ücretsiz deneme sürümünü başlatın.'
JoVE Journal Kimya
Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

DOI:
10.3791/53644-v

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09:05 min

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January 06, 2016

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,
1'Kimya Bölümü',University at Albany, State University of New York, 2Multiplex Biotechnology Laboratory,Cancer Research Center

'Bölümler'

  • 00:05'Başlık'
  • 00:44One-dimensional DNA Barcode Slides: Preparing the PDMS Barcode Mold
  • 02:21One-dimensional DNA Barcode Slides: Patterning of Poly-L-lysine (PLL)
  • 03:39One-dimensional DNA Barcode Slides: Patterning of ssDNA
  • 05:00Two-dimensional 3×3 DNA Arrays: Preparing the PDMS Barcode Mold and Flow Patterning of ssDNA
  • 06:17Two-dimensional 3×3 DNA Arrays: Validation and Immunoassay
  • 07:32Results: Multiplex-protein Detection from Single-cell Preparations
  • 08:25Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

This protocol outlines the fabrication of a large-scale, multiplexed two-dimensional DNA or antibody array, with potential applications in cell signaling studies and biomarker detection.

'Etiketler'

Flow-patternBarcodeAntibody MicroarraySingle-cell AnalysisPDMS MoldSU-8 MasterPhoto-maskPoly-L-lysinePolyethylene TubingStainless Steel Pins

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