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JoVE Journal Chemistry
Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
 

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Article DOI: 10.3791/53644-v • 09:05 min • January 6th, 2016
January 6th, 2016 •
1,2, 1,2
1Department of Chemistry, University at Albany, State University of New York, 2Multiplex Biotechnology Laboratory, Cancer Research Center

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Summary
Automatic Translation

This protocol outlines the fabrication of a large-scale, multiplexed two-dimensional DNA or antibody array, with potential applications in cell signaling studies and biomarker detection.

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Flow-pattern Guided Fabrication High-density Barcode Antibody Microarray Single-cell Analysis Detect Secret Proteins Intracellular Proteins Micro-array Platform Customizable Technique Flow Patterning Strategies SU-8 Master Chrome Photo-mask Photo-resist Coat UV Pattern Application PDMS Barcode Mold Preparation Silicon Elastomer Base Curing Agent De-gas Pre-polymer Solution Petri Dish Silicon Wafer Depth Of 7.45 Millimeters De-gas Mixture Baking At 75 Degrees Celsius To Cure PDMS
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