'Dergi'
/
Çevre
/
A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
JoVE Journal
Çevre
'Bu içeriği görüntülemek için JoVE aboneliği gereklidir.'  'Oturum açın veya ücretsiz deneme sürümünü başlatın.'
JoVE Journal Çevre
A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

DOI:
10.3791/53611-v

•

12:14 min

•

March 09, 2016

•

, ,
1Department of Microbiology,Southern California Costal Water Research Project Authority

'Bölümler'

  • 00:05'Başlık'
  • 00:56Assay Mixture Preparation
  • 02:24Droplet Generation and PCR Plate Setup
  • 05:17Thermal Cycling and Droplet Reading
  • 08:01Data Analysis and Reporting
  • 09:46Results: Duplex Digital PCR is a Robust Assay for Simultaneous Quantification of General and Human-associated Fecal Contamination
  • 11:18Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

This manuscript describes a duplex digital PCR assay that can be used to simultaneously quantify Enterococcus spp. and the HF183 genetic markers as indicators of general and human-associated fecal contamination in recreational waters.

'Etiketler'

Duplex Digital PCR AssayEnterococcus Spp.HF183 MarkerFecal ContaminationWater Quality MonitoringQPCRMaster MixDroplet GenerationPositive ControlsNo Template Controls (NTCs)

Article

Embed

'ÇALMA LİSTESİNE EKLE'

Usage Statistics

'İlgili Videolar'

Determination of Microbial Extracellular Enzyme Activity in Waters, Soils, and Sediments using High Throughput Microplate Assays

Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies

Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis

Extraction of Organochlorine Pesticides from Plastic Pellets and Plastic Type Analysis

The Hawaii Protocol for Scientific Monitoring of Coffee Berry Borer: a Model for Coffee Agroecosystems Worldwide

Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples

Measuring the Flight Ability of the Ambrosia Beetle, Platypus Quercivorus (Murayama), Using a Low-Cost, Small, and Easily Constructed Flight Mill

Harvesting Venom Toxins from Assassin Bugs and Other Heteropteran Insects

Read Article