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伤口划痕试验的优化来探测在小鼠间充质干细胞迁移的可溶性香烟烟雾提取物受损后
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JoVE Journal Gelişim Biyolojisi
Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract
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伤口划痕试验的优化来探测在小鼠间充质干细胞迁移的可溶性香烟烟雾提取物受损后

16,749 Views

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08:40 min

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December 03, 2015

DOI:

10.3791/53414-v

DOI:
10.3791/53414-v

•

08:40 min
December 03, 2015

•

2 Views
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1Biyoloji Bölümü,College of the Holy Cross

Özet

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The capacity to migrate is a key function of many different cell types, including mesenchymal stromal cells (MSCs). However, quantifying alterations in migratory capacity after damage is challenging. This protocol describes an easily adaptable migration assay that uses rigorous statistics to quantify changes in MSC migratory capacity after damage.

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Optimized Wound Scratch AssayMesenchymal Stromal Cell MigrationQuantitative Migration AnalysisSoluble Cigarette Smoke ExtractCell Migration AssayScratch Assay OptimizationBoyden ChamberCell Exclusion Zone AssayCell Migration QuantificationReproducibilityCustomizabilityCost-effectiveness

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Cite this Article

Cormier, N., Yeo, A., Fiorentino, E., Paxson, J. Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract. J. Vis. Exp. (106), e53414, doi:10.3791/53414 (2015).

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