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Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract

Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract

DOI:
10.3791/53414-v

•

08:40 min

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December 03, 2015

•

, , ,
1Biyoloji Bölümü,College of the Holy Cross

Bölümler

  • 00:05Başlık
  • 00:40Creating the Scratch
  • 02:51Taking Initial Images of the Scratch
  • 04:34Analyzing Initial Scratch Images
  • 06:07Taking Final Images of the Scratch and Analyzing Final Scratch Images
  • 06:52Results: Migratory of Murine Lung MSCs is Altered After Damage with Cigarette Smoke Extract
  • 08:02Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

The capacity to migrate is a key function of many different cell types, including mesenchymal stromal cells (MSCs). However, quantifying alterations in migratory capacity after damage is challenging. This protocol describes an easily adaptable migration assay that uses rigorous statistics to quantify changes in MSC migratory capacity after damage.

Etiketler

Optimized Wound Scratch AssayMesenchymal Stromal Cell MigrationQuantitative Migration AnalysisSoluble Cigarette Smoke ExtractCell Migration AssayScratch Assay OptimizationBoyden ChamberCell Exclusion Zone AssayCell Migration QuantificationReproducibilityCustomizabilityCost-effectiveness

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