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Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract

Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract

16,733 Views

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08:40 min

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December 03, 2015

DOI:

10.3791/53414-v

DOI:
10.3791/53414-v

•

08:40 min
December 03, 2015

•

3 Views
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1Department of Biology,College of the Holy Cross

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Optimized Wound Scratch AssayMesenchymal Stromal Cell MigrationQuantitative Migration AnalysisSoluble Cigarette Smoke ExtractCell Migration AssayScratch Assay OptimizationBoyden ChamberCell Exclusion Zone AssayCell Migration QuantificationReproducibilityCustomizabilityCost-effectiveness

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Cormier, N., Yeo, A., Fiorentino, E., Paxson, J. Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract. J. Vis. Exp. (106), e53414, doi:10.3791/53414 (2015).

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