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חלופה פשוטה להזרקת stereotactic למציאה ספציפית המוח של מירנה
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JoVE Journal Nörobilim
A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA
Please note that all translations are automatically generated. Click here for the English version.

חלופה פשוטה להזרקת stereotactic למציאה ספציפית המוח של מירנה

12,551 Views

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06:53 min

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December 26, 2015

DOI:

10.3791/53307-v

DOI:
10.3791/53307-v

•

06:53 min
December 26, 2015

•

6 Views
, , , , , ,
1CSIR-Institute of Genomics and Integrative Biology, New Delhi, India, 2Academy of Scientific and Innovative Research (AcSIR), New Delhi, India

Özet

Automatically generated

MicroRNAs play crucial roles in the brain and are potential targets for modeling neuro-degeneration. However, perturbing miRNA levels is challenging due to the short length of miRNA and inaccessibility of the brain tissue. This video presents a method for antagomir design and brain specific delivery using a neuropeptide in mice.

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MiRNAMicroRNAAntagomirLNARVGBrain DeliveryStereotactic InjectionNeurodegenerative DiseaseNeuronal Cell DeathGene ExpressionNeurodevelopmentNeuronal Function

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Suryawanshi, H., Sarangdhar, M. A., Vij, M., Roshan, R., Singh, V. P., Ganguli, M., Pillai, B. A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA. J. Vis. Exp. (106), e53307, doi:10.3791/53307 (2015).

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