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Methoden te remmen Bacteriële Pyomelanin Productie en Bepaal de overeenkomstige stijging van de gevoeligheid voor oxidatieve stress
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress

Methoden te remmen Bacteriële Pyomelanin Productie en Bepaal de overeenkomstige stijging van de gevoeligheid voor oxidatieve stress

DOI:

11:00 min

August 31, 2015

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Bölümler

  • 00:05Başlık
  • 01:47Antibiotic Minimum Inhibitory Concentration (MIC) Assay in 96-well Plates
  • 05:33Spot Plate Assay for Oxidative Stress Response
  • 09:11Results: Inhibition of Pyomelanin Production Increases Sensitiity to Oxidative Stress
  • 10:32Conclusion

Özet

Otomatik Çeviri

Bacterial pyomelanin production results in increased resistance to oxidative stress and virulence. We report on techniques that can be used to determine inhibition of pyomelanin production and assay the resulting increase in sensitivity to oxidative stress in bacteria, as well as determine antibiotic minimum inhibitory concentration (MIC).

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