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Immunology and Infection

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JoVE Journal Immunology and Infection
Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress
 

Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress

Article DOI: 10.3791/53105-v • 11:00 min • August 31st, 2015
August 31st, 2015 •
1, 1
1Department of Biological Sciences, University of Wisconsin Milwaukee

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Bacterial pyomelanin production results in increased resistance to oxidative stress and virulence. We report on techniques that can be used to determine inhibition of pyomelanin production and assay the resulting increase in sensitivity to oxidative stress in bacteria, as well as determine antibiotic minimum inhibitory concentration (MIC).

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Inhibit Bacterial Pyomelanin Production Increase Sensitivity To Oxidative Stress Red-brown Pigment Tyrosine Catabolism Pathway Pseudomonas Aeruginosa 2-[2-nitro-4-(trifluoromethyl)benzoyl]-1,3-cyclohexanedione (NTBC) Titration Method Drug Concentration Pigment Production Reduction Microtiter Plate Method Antibiotic Minimum Inhibitory Concentration (MIC) Bacterial Growth Inhibition Non-antibiotic Compounds Bacterial Sensitivity To Oxidative Stress H2O2 Agar Plates
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