Using multiple angles to cut the mouse pup brain, we improve upon a previously-described acute brain slice which captures the connections between most of the major auditory midbrain and forebrain structures.
Slater, B. J., Fan, A. Y., Stebbings, K. A., Saif, M. T. A., Llano, D. A. Modification of a Colliculo-thalamocortical Mouse Brain Slice, Incorporating 3-D printing of Chamber Components and Multi-scale Optical Imaging. J. Vis. Exp. (103), e53067, doi:10.3791/53067 (2015).