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Modification of a Colliculo-thalamocortical Mouse Brain Slice, Incorporating 3-D printing of Chamber Components and Multi-scale Optical Imaging
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JoVE Journal Neuroscienze
Modification of a Colliculo-thalamocortical Mouse Brain Slice, Incorporating 3-D printing of Chamber Components and Multi-scale Optical Imaging

Modification of a Colliculo-thalamocortical Mouse Brain Slice, Incorporating 3-D printing of Chamber Components and Multi-scale Optical Imaging

DOI:
10.3791/53067-v

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06:05 min

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September 18, 2015

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, , , ,
1Neuroscience Program,University of Illinois Urbana-Champaign, 2Mechanical Science and Engineering,University of Illinois Urbana-Champaign, 3Molecular and Integrative Physiology,University of Illinois Urbana-Champaign

Capitoli

  • 00:05Titolo
  • 01:14Preparation for Brain Slicing
  • 02:57Obtaining the Colliculo-thalamocortical Slice
  • 03:51Imaging of the Slice
  • 04:38Results: Flavoprotein Autofluorescence Imaging of Colliculo-thalamocortical Brain Slice
  • 05:42Conclusion

Summary

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Using multiple angles to cut the mouse pup brain, we improve upon a previously-described acute brain slice which captures the connections between most of the major auditory midbrain and forebrain structures.

Tags

Keywords: Brain SliceAuditory SystemInferior ColliculusMedial Geniculate BodyThalamic Reticular NucleusAuditory CortexFlavoprotein Autofluorescence ImagingConnectivityInformation Processing3D PrintingSlice Chamber

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