/
/
Aislamiento de leucocitos a partir de los tejidos murinos en la interfase materno-fetal
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Aislamiento de leucocitos a partir de los tejidos murinos en la interfase materno-fetal
16,806 Views
•
07:51 min
•
May 21, 2015
DOI:
1Department of Obstetrics & Gynecology,Wayne Eyalet Üniversitesi Tıp Fakültesi, 2School of Paediatrics and Reproductive Health, Research Centre for Reproductive Health, the Robinson Research Institute,The University of Adelaide, 3Department of Immunology & Microbiology,Wayne Eyalet Üniversitesi Tıp Fakültesi, 4Perinatology Research Branch,NICHD/NIH/DHHS
Özet
Automatically generated
Described herein is a protocol to isolate and analyze the infiltrating leukocytes of tissues at the maternal-fetal interface (uterus, decidua, and placenta) of mice. This protocol maintains the integrity of most cell surface markers and yields enough viable cells for downstream applications including flow cytometry analysis.
Read Article
Cite this Article
Arenas-Hernandez, M., Sanchez-Rodriguez, E. N., Mial, T. N., Robertson, S. A., Gomez-Lopez, N. Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface. J. Vis. Exp. (99), e52866, doi:10.3791/52866 (2015).
Copy